IMR Press / CEOG / Volume 49 / Issue 8 / DOI: 10.31083/j.ceog4908182
Open Access Original Research
Predictive Value of Tissue-induced Oxidative Stress on Urogynecology Synthetic Graft Reinforcement: An Experimental Animal Study
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1 Surgery Department, Medical faculty University of Nis, 18000 Nis, Serbia
2 Clinic of Urology, Clinical Center of Nis, 18000 Nis, Serbia
*Correspondence: uropota@gmail.com (Potic Milan)
These authors contributed equally.
Academic Editor: Michael H. Dahan and Shigeki Matsubara
Clin. Exp. Obstet. Gynecol. 2022, 49(8), 182; https://doi.org/10.31083/j.ceog4908182
Submitted: 18 February 2022 | Revised: 23 April 2022 | Accepted: 25 April 2022 | Published: 2 August 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Background: The success of urogynecology synthetic grafts depends on adequate tissue reinforcement. This experimental animal study aimed to determine the abdominal wall reinforcement achieved by different urogynecology synthetic grafts, including the influence of inflammatory cells, collagen deposits, and tissue-induced oxidative stress. Methods: Electron microscopic analysis of six different grafts, all with Polypropylene as their major component, was performed to determine the primary mesh characteristics. Full-thickness abdominal wall defects were repaired using monofilament, multifilament, and coated grafts in male Wistar rats. After six weeks, the animals were sacrificed and the inflammatory response, collagen deposition, and oxidative stress levels were quantified. Using the digital acquisition system (Hottinger Baldwin Messetechnik (HBM) “Catman Easy”, Darmstadt, Germany), mechanical testing of the native grafts and of the reinforced abdominal wall was conducted and measured in a controlled environment. Multivariate analysis was performed to determine the predictive value of inflammatory cell numbers, collagen amount, oxidative stress, and native graft strength on the final abdominal wall reinforcement. Results: The inflammatory response was significantly more prominent with the multifilament polypropylene compared to the low-weight monofilament polypropylene (p < 0.05). Collagen deposits varied between the groups, reaching statistical significance only for multifilament polypropylene vs. titanium-coated polypropylene (p < 0.05). The oxidative stress results demonstrated a positive correlation with graft weight, regardless of coating or different graft structures (p < 0.05). The number of inflammatory cells and collagen amount did not influence the final abdominal reinforcement, while tissue-induced oxidative stress presented with a negative influence in all groups. Conclusions: Tissue-induced oxidative stress negatively affected grafts in this animal experiment. This finding might be useful (at least partially) in predicting the effectiveness of urogynecology synthetic graft tissue reinforcement and also, in promoting this reinforcement.

Keywords
inflammation
mesh
oxidative stress
mechanical strength
collagen quantification
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