Background and Objective: Atherosclerosis is the major cause of death in developing country and complete management of it was not able to achieve with the available treatment options. Thus, present study evaluates the anti-atherosclerosis activity of Murraya exotica (ME) in hypercholesterolemic rats. Materials and Methods: Hypercholesterolemia was induced by feeding atherosclerosis feed to the rats for the period of 4 weeks and rats were treated with ME 100 and 200 mg kg–1 during the induction of hypercholesterolemia. Body weight, food and water intake was estimated every week till the end of protocol. However, at the end of protocol lipid profile in the blood and markers of liver and endothelial function and oxidative stress parameters were assessed in tissue homogenate. Results: Data of this study suggested that treatment with ME significantly decreases the percentage gain in the body weight of rat and food intake than negative control group. It was also observed that altered level of lipid profile get attenuated in ME treated group. In addition treatment with ME attenuates the altered level of oxidative stress parameters and markers of endothelial and liver dysfunction in hypercholesterolemic rats. Moreover, ME attenuates the markers of endothelial dysfunction in hypercholesterolemic rats. Conclusion: This study revealed that treatment with ME produces anti-atherosclerogenic activity on the basis of its hyperlipidemic and antioxidant effect in hypercholesterolemic rats.