Background and objective: Angiopoietin-like protein 2 (ANGPTL2) is a pro-inflammatory adipokine that is upregulated in obesity and plays a role in the progression of cardiometabolic diseases, including diabetes and atherosclerosis. Aerobic exercise is one of the effective strategies for reducing the levels of various pro-inflammatory biomolecules in obese individuals. However, the effects of aerobic exercise training on circulating ANGPTL2 levels in obese individuals remain unclear. The objective of this study was to investigate the effect of aerobic exercise training on serum ANGPTL2 levels in overweight and obese men. Material and methods: Twenty overweight and obese men (age, 49 \pm10 years; body mass index, 27.4 \pm 2.2 kg/m{}^2) completed a 12-week aerobic exercise training program (60–85% Heart rate{}_{max}, 40–60 min/day, 3 days/week). Before and after the exercise program, serum ANGPTL2 levels were measured using the enzyme-linked immunosorbent assay. Daily step counts and the different physical activities based on the intensity were assessed using a triaxial accelerometer. Results: Serum ANGPTL2 levels were significantly decreased after the 12-week aerobic exercise training program ((3.0 \pm 0.6) vs. (2.7 \pm 0.7) ng/mL, P 0.05). Daily step counts ((8362 \pm 4551) vs. (10357 \pm 3168) steps/day, P 0.05) and moderate- to vigorous-intensity physical activity (MVPA) time ((58 \pm 45) vs. (76 \pm 37) min/day, P 0.001) were significantly increased after the exercise intervention. The changes in serum ANGPTL2 levels were negatively correlated with corresponding changes in daily step counts (partial r = –0.49, P 0.05) and MVPA time (partial r = –0.47, P 0.05) after adjustment for age and accelerometer wear time. Conlcusion: These findings collectively suggest that aerobic exercise training, in particular an increase in MVPA, can be associated with decreased circulating levels of ANGPTL2 in overweight and obese men.