Background: Physical inactivity is one of the greatest problems facing the world today. The purpose of this study was to examine the level of barriers to physical activity for middle-aged adults during the COVID-19. Methods: Using an online survey, a sample of the Turkish population (n = 432: 48.6% female, 51.4% male; mean age 57.3 years) answered questions about sociodemographic characteristics as well as barriers to participation in physical activity during COVID-19 process. A scale of barriers to physical activity was used to determine the barriers to physical activity faced by the participants COVID-19 process. Since the data were homogeneously distributed across binary groups, independent t-tests and ANOVAs were performed for groups of three or more. In addition, if there were significant differences in the results of the ANOVA, Tukey’s post hoc test was also applied. The data were analyzed using SPSS Version 22.0. (IBM Corp., Armonk, NY, USA). Results: According to the findings of this study, barriers to physical activity increase significantly as perceived health status decreases, and women’s barriers to physical activity, participation are greater than those of men, and increase with age. At the same time, the strongest relationship was found for environmental factors. In addition, important results were found for the personal and social environment dimensions, and we also found that barriers to physical activity increase significantly with age. Conclusions: Finally, we determined that the barriers to physical activity for individuals with COVID-19 were significantly greater at the personal level compared to those who did not have COVID-19. That is the COVID-19 process negatively affected participation in physical activity.