ATF3 inhibits the growth and stem cells-like features of SW620 colorectal cancer cells in vitro

Chuanqian Huang; Changdan Chen; Fangjing Zheng; Xiaoxiao Ni; Jianfang Lin; Wenjuan Wu; Xiaolan Lai

doi:10.31083/jomh.2021.072

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IMR Press / JOMH / Volume 17 / Issue 4 / DOI: 10.31083/jomh.2021.072

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ATF3 inhibits the growth and stem cells-like features of SW620 colorectal cancer cells in vitro

Chuanqian Huang¹, Changdan Chen², Fangjing Zheng¹, Xiaoxiao Ni¹, Jianfang Lin¹, Wenjuan Wu¹, Xiaolan Lai^3,*

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¹ Department of Medical Oncology and Radiotherapy, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China

² Department of Gastroenterology, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China

³ Department of Hematology and Rheumatism, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China

^*Correspondence: drxiaolan@163.com (Xiaolan Lai)

J. Mens. Health 2021, 17(4), 287–294; https://doi.org/10.31083/jomh.2021.072

Submitted: 6 April 2021 | Accepted: 8 June 2021 | Published: 30 September 2021

This is an open access article under the CC BY 4.0 license (https://creativecommons.org/licenses/by/4.0/).

Abstract

Background and objective: Activating transcription factor 3 (ATF3) plays a crucial role in regulating tumor development depending on the cellular context or cancer cell type. However, the effect of ATF3 on stem cells-like features in colorectal cancer (CRC) has yet to be elaborated.

Methods: In this study, we overexpressed ATF3 in SW620 CRC cells to investigate its effects on stem cells-like features.

Results: Our results indicated that overexpressing ATF3 inhibited the proliferation, invasion, migration, and sphere formation capacity of SW620 CRC cells. ATF3 overexpression also decreased the size of tumorspheres and reduced expression of the cancer stem cell markers CD44 and CD133 in SW620 cells.

Conclusion: In summary, our study revealed that ATF3 suppresses CRC growth and stem cells-like features. ATF3 is considered a potential target in CRC therapy.

Keywords

ATF3

Migration

Invasion

Cancer stem cells

Funding

2018J05151/Natural Science of Foundation of Fujian Province

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Fig. 1.

Expression of ATF3 in colorectal cell lines. (A) RT-PCR was used to assess ATF3 mRNA levels in normal colon cells and colorectal cancer (CRC) cell lines. **p $<$ 0.01, ***p $<$ 0.001, NS = not significant, compared with other CRC cell lines and NCM460 cells by independent Student’s t test. (B) ATF3 overexpression in SW620 cells was confirmed by western blot analysis. Ctrl, control; OE, overexpression.

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J. Mens. Health Print ISSN 1875-6867 Electronic ISSN 1875-6859