NSD1 stimulated survival and migration of gastric cancer cells through WNT10B

Background and objective: To assess the expression of Nuclear receptor binding SET domain protein 1 (NSD1) in human gastric cancer tissues and cells and investigate its possible role in gastric cancer.

Methods: TCGA database was used to assess the expression levels of NSD1 in human gastric cancer tissues. Immunoblot assays were performed to detect NSD1 expression levels in gastric cancer cell lines. MTT and colony formation assays were conduced to detect its role in the survival of gastric cancer cells. Wound closure and transwell were performed to investigate the effects of NSD1 on the motility of gastric cancer cells. Immunoblot assays were also conducted to confirm its effects on WNT10B/ $\beta$ -catenin pathway.

Results: We found the high expression levels of NSD1 in human gastric cancer tissues and cell lines. NSD1 depletion suppressed the survival and motility of gastric cancer cells. Additionally, we revealed NSD1 activated the WNT10B/ $\beta$ -catenin pathway, therefore promoted gastric cancer progression.

Conclusion: We revealed the high NSD1 expression in gastric cancer tissues and cells, and thought NSD1 could serve as a promising gastric cancer target.

Keywords

Nuclear receptor binding SET domain protein 1 (NSD1)

Gastric cancer

Survival

Motility

WNT10B/

\beta

-catenin

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Fig. 1.

NSD1 was highly expressed in human GC tissues and cell lines. (a) TCGA database showed the comparison of the transcript per million of NSD1 between human GC tissues and normal tissues. (b) Immunoblot assays revealed that NSD1 was overexpressed in AGS, MKN45, and HGC-27 cell lines than that in GES-1 cell line. Data were presented as mean $\pm{}$ SEM, *** p $<$ 0.001.

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J. Mens. Health Print ISSN 1875-6867 Electronic ISSN 1875-6859