IMR Press / JOMH / Volume 14 / Issue 4 / DOI: 10.22374/1875-6859.14.4.4

Journal of Men’s Health (JOMH) is published by IMR Press from Volume 17 Issue 1 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Dougmar Publishing Group.

Original Research

EPIDEMIOLOGICAL STUDY OF CUTANEOUS LEISHMANIASIS IN SOME IRAQI PROVINCES

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1 Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Science (TUMS-IC), Tehran, Iran
2 Department of Microbiology, College of Medicine, Wasit University, Kut, Iraq
3 Department of Biology, College of Science, Wasit University, Kut, Iraq
4 Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5 Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
6 Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

*Author to whom correspondence should be addressed.

J. Mens. Health 2018, 14(4), 18–24; https://doi.org/10.22374/1875-6859.14.4.4
Submitted: 17 August 2018 | Accepted: 27 September 2018 | Published: 11 October 2018
Abstract

Background

Cutaneous leishmaniasis (CL) remains a serious public health concern in some parts of Iraq. The aims of this study to report of CL in some parts of Iraq, by different parasitological, cultural, and molecular meth-ods and evaluate sex differences among infected patients. This is the first study conducted to characterize Leishmania species causing CL among Iraqi patients using the sequence analysis of Internal Transcribed Spacer1(ITS1) at Wasit Province.

Methods

A total of 700 cases of suspected CL were referred to the Iraqi clinics and health centres and they checked for Leishmania amastigote using a Giemsa-stain; however, the Novy Macneal Nicolle (NNN) culture led to the growth of promastigotes in all samples, then the samples were examined using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP)-PCR methods.

Results

The present study indicated that the prevalence of CL as follow: AL-Diwaniyah 88(15.1%), Wasit 85 (14.5%), Najaf 79 (13.6%), Thi-Qar76 (13.1%), Basrah 67 (11.5%), Baghdad 65 (11.2%), Diyala 63(10.8%), and Salah-Edin province which recorded lower infection 60 (10.3%), and it appeared in 83.3% by using Giemsa-smeared stain. The highest infection rate (100%) appeared using PCR while the lowest infection (68%) appeared by culture on NNN media. The present study was revealed that the highest infection (60%) was caused by L.major rather than L.tropica (40%). Our results showed that 368 (52.6%) of CL patients were had single lesion and 215 (30.7%) had multiple lesions, and the ulcerative wet type lesions were present in 49.6%, while the nodule dry type lesions were present in 33.7%. The overall prevalence of cutaneous leishmaniasis in the study area was very high (83.3%) having a statistically significant association with sex; males are more prone (56.4%) to CL as compared to females (43.6%).

Conclusions

A clear and reliable bias toward males exists in some tropical diseases, such as leishmaniasis. CL is a major health problem in Iraq and CL caused by many countries including Iraq. Health authorities should be aware of the fact that war and terrorist activities induce expansion of the disease and increase the incidence rate in the situation that access to medical treatment is not easy especially in poor conditions in leishmaniasis endemic areas.

Keywords
Cutaneous leishmaniasis
Gender
Molecular
Human
Iraq
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