IMR Press / JIN / Volume 20 / Issue 1 / DOI: 10.31083/j.jin.2021.01.254
Open Access Original Research
Broad and dynamic neurochemical alterations in the brain of alcoholic rats
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1 Molecular Medical Laboratory, Institute of Life Science, Chongqing Medical University, 400016 Chongqing, P. R. China
2 Department of Cerebrovascular Diseases, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101 Yunnan Province, P. R. China
3 Department of Anesthesiology, Guizhou Provincial People’s Hospital, Guiyang, 550002 Guizhou Province, P. R. China
*Correspondence: (Fei-Fei Shang)
These authors contributed equally.
J. Integr. Neurosci. 2021, 20(1), 33–41;
Submitted: 23 August 2020 | Revised: 30 November 2020 | Accepted: 3 December 2020 | Published: 30 March 2021
Copyright: © 2021 The Authors. Published by IMR Press.
This is an open access article under the CC BY 4.0 license (

Ethanol is the active ingredient in alcoholic beverages. As ethanol consumption increases from zero to very high, it is still unknown which metabolites are present at different times and which are essential to normal functioning. In this article, we used an intermittent-access 20% ethanol drinking paradigm to make Wistar male rats voluntarily drink large amounts of ethanol for 10, 20, 30, and 50 days, respectively. A hydrogen-1 nuclear magnetic resonance approach was used to investigate the time-dependent neurochemical metabolites spectra in the hippocampus, striatum, nucleus accumbens and prefrontal cortex. Multivariate pattern recognition techniques were used to analyze the hydrogen-1 nuclear magnetic resonance spectra data. Metabolic profiling was obtained, differentiating the ethanol-treated and control rats. The ethanol-affected metabolites disrupted processes associated with neurotransmitters, oxidative stress, energy metabolism and amino acids. Together, our findings demonstrate broad, dynamic, and time-dependent endogenous metabolic alterations in rats treated with ethanol.

Ethanol consumption
Nucleus accumbens
Prefrontal cortex
Fig. 1.
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