Phosphorus-31 nuclear-spin entanglements within Ca
molecules (Posner molecules) have been proposed to be central for neural processing. However, this has yet to be proven experimentally. Relatedly, increasing calcium ion concentration in the cerebrospinal fluid has been proposed to enhance consciousness by accelerating Posner molecules’ creation. A dependence on calcium isotope is also expected. Here we test these predictions experimentally by measuring the loss of righting reflex ED
for mice to sevoflurane - an increase in loss of righting reflex ED
indicates a higher level of consciousness and vice versa. Our mice’s findings demonstrate that intracerebroventricular injection of EGTA enhances the sevoflurane-induced loss of righting reflex ED
while injecting calcium-40 chloride or calcium-43 chloride causes an opposite effect. Further, the identical effects of calcium-40 and calcium-43 indicate an absence of calcium isotope dependence. Here, our findings disprove conventional proposals that calcium ion concentration correlates with consciousness.