IMR Press / JIN / Volume 19 / Issue 3 / DOI: 10.31083/j.jin.2020.03.002
Open Access Rapid Report
Herbal decoction of Gastrodia, Uncaria, and Curcuma confers neuroprotection against cerebral ischemia in vitro and in vivo
Show Less
1 Department of Neurosurgery, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, P. R. China
2 Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, P. R. China
*Correspondence: (Zi-Long Wei); (Li Ren)
These authors contributed equally.
J. Integr. Neurosci. 2020, 19(3), 513–519;
Submitted: 6 January 2020 | Revised: 16 April 2020 | Accepted: 25 April 2020 | Published: 30 September 2020
Copyright: © 2020 Wang et al. Published by IMR Press.
This is an open access article under the CC BY-NC 4.0 license (

“Tianma” (Gastrodia) and “gouteng” (Uncaria) are both widely used to treat cerebral ischemia. At the same time, “ezhu” (Curcuma longa) or turmeric, is derived from the dried roots of C. longa. It is a polyphenol known for its anti-inflammatory effects and its promotion of blood vessel endothelial function. This study explored the neuroprotective effects of a water extract of “tianma”, “gouteng”, and “ezhu” against ischemic injury. Flow cytometry analysis showed that Gastrodia, Uncaria, and Curcuma reduced the proportion of apoptotic cells in CoCl2 induced B35 (P = 0.0027) and SH-SY5Y (P = 0.0006) cell sample relative to the respective control group. Western blot indicated that Gastrodia, Uncaria, and Curcuma upregulated the expression of Bcl-2 and inversely downregulated Bax and Caspase-3 (P< 0.001). The infarct volume observed in the Gastrodia, Uncaria, and Curcuma group was also decreased compared with the control group (P < 0.05). Immunofluorescence detection revealed a lower expression of Caspase-7 in the Gastrodia, Uncaria, and Curcuma group than in the control group, while expression was negligible in the sham group. Gastrodia, Uncaria, and Curcuma confer neuroprotective effects in CoCl2 induced B35/SH-SY5Y cells and a rat model of ischemia by way of its anti-apoptotic effects.

Alternative medicine
Chinese herbal medicine
cerebral ischemia
Fig. 1.
Back to top