IMR Press / JIN / Volume 18 / Issue 2 / DOI: 10.31083/j.jin.2019.02.16
Open Access Original Research
Hippocampal neuron loss and astrogliosis in medial temporal lobe epileptic patients with mental disorders
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1 Department of Neurosurgery, Hunan Provincial Brain Hospital, 410007, P. R. China
2 Department of Neurology, Central South University, 410000, P. R. China
3 Department of Pathology, Hunan Provincial Brain Hospital, 410007, P. R. China
4 Department of Psychiatry, Hunan Provincial Brain Hospital, 410007, P. R. China
5 Department of Neurology, Hunan Provincial Brain Hospital, 410007, P. R. China
*Correspondence: lujundsa@163.com (Jun Lu)
J. Integr. Neurosci. 2019, 18(2), 127–132; https://doi.org/10.31083/j.jin.2019.02.16
Submitted: 19 January 2019 | Accepted: 25 April 2019 | Published: 30 June 2019
Copyright: © 2019 Lu et al. Published by IMR press.
This is an open access article under the CC BY-NC 4.0 license (https://creativecommons.org/licenses/by/4.0/)
Abstract

Hippocampal neuron loss and reactive astrogliosis are pathological features of medial temporal lobe epilepsy. Here, the expression of hippocampal astrogliosis-associated genes are studied in subjects with medial temporal lobe epilepsy and mental disorders (such as depression, anxiety and psychiatric comorbidities). The relationship between functional changes in hippocampus astrocytes and concurrent mental disorders are discussed. Nissl staining identified medial temporal lobe epilepsyinduced neuronal loss in the CA1 region of hippocampus. Quantitative real-time polymerase chain reaction and immunofluorescence technology were used to detect hippocampus glial fibrillary acidic protein, metallothionein, and aquaporin-4. The hippocampus area of subjects with medial temporal lobe epilepsy (with or without mental disorders) were smaller than the control group. Hippocampal neuronal loss and astrogliosis were more obvious in groups of medial temporal lobe epileptic patients with mental disorders. Relative protein levels of glial fibrillary acidic protein, metallothionein-I/II, and aquaporin-4 were significantly higher in subjects with medial temporal lobe epilepsy than seen in controls. Medial temporal lobe epileptic patients with mental disorder or depression had elevated metallothionein-I/II protein level when compared to controls and medial temporal lobe epileptic patients without mental disorder. Protein levels of glial fibrillary acidic protein and aquaporin-4 in medial temporal lobe epileptic patients with mental disorders were significantly lower than that in medial temporal lobe epileptic patients with no mental disorder. It is concluded that functional changes in hippocampus astrocytes are associated with mental disorders in medial temporal lobe epileptic patients and the astrogliosis-related genes of glial fibrillary acidic protein, metallothionein-I/II and aquaporin-4, are involved in this process.

Keywords
Medial temporal lobe epilepsy
mental disorder
astrocytes
glial fibrillary acidic protein
metallothionein-I/II
aquaporin-4
Figures
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