IMR Press / JIN / Volume 18 / Issue 2 / DOI: 10.31083/j.jin.2019.02.145
Open Access Original Research
Protein kinase C-ε contributes to a chronic inhibitory effect of IL-1β on voltage-gated sodium channels in mice with febrile seizure
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1 Department of Pediatrics, Jinan Central Hospital Affiliated to Shandong University, Jinan City, Shandong Province, 250013, P. R. China
2 Graduate School of Taishan Medical University, Taian City, Shandong Province, 271016, P. R. China
*Correspondence: zhy1587@zxyy.cn (Hongyang Zhao)
J. Integr. Neurosci. 2019, 18(2), 173–179; https://doi.org/10.31083/j.jin.2019.02.145
Submitted: 26 March 2019 | Accepted: 26 June 2019 | Published: 30 June 2019
Copyright: © 2019 Wang et al. Published by IMR press.
This is an open access article under the CC BY-NC 4.0 license (https://creativecommons.org/licenses/by-nc/4.0/)
Abstract

This study aimed to understand the role of Interleukin-1β in mouse febrile seizures. To investigate the chronic effects of raised Interleukin-1β on seizures, the sodium currents of hippocampal neurons were recorded by whole-cell voltage clamp. Interleukin-1β inhibited sodium currents in mouse hippocampal neurons and verified that protein kinase C epsilon contributed to the effect of Interleukin-1β exposure. The inhibitory effect was also identified in neurons from a protein kinase C epsilon null mutant mouse. Action potentials were recorded using a ramp depolarizing current. Peak spike depolarization was significantly reduced by Interleukin-1β treatment, and was abolished following the administration of a protein kinase C epsilon inhibitor, εV1-2. However, neither Interleukin-1β nor εV12 had any significant effect on spike threshold. Interleukin1β reduced the amplitude of action potentials due to its inhibitory effect on sodium channels. This is hypothesised to decrease the release of presynaptic transmitters of neuroexcitability, thus exerting a neuroprotective role in excitotoxicity. To ascertain the role of protein kinase C epsilon on febrile seizures in vivo, a heated water-bath model was used to identify susceptible mice. It was found that protein kinase C epsilon reduced susceptibility to, and frequency of, febrile seizure onset. This may be related to the neuroprotective effect of Interleukin-1β on hippocampal neurons.

Keywords
Febrile seizure
IL-1β
hippocampus
voltage-gated sodium channel
PKC-ε
Figures
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