IMR Press / JIN / Volume 18 / Issue 1 / DOI: 10.31083/j.jin.2019.01.118
Open Access Original Research
Voluntary exercise promotes neurotrophic factor and suppresses apoptosis in hippocampal ischemia
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1 Department of Neurology, The 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army, No.333 Binhe Road, Qilihe District, Lanzhou City, Gansu Province, 730050, China
*Correspondence: (Rong Yin)
# These authors contributed equally.
J. Integr. Neurosci. 2019, 18(1), 65–70;
Submitted: 24 December 2018 | Accepted: 15 March 2019 | Published: 30 March 2019
Copyright: © 2019 Zhang et al. Published by IMR press.
This is an open access article under the CC BY-NC 4.0 license (

Previous studies have demonstrated that exercise facilitates recovery from ischemia. However, the mechanisms need to be further elucidated. The current investigation was designed to study the effect of voluntary exercise on cerebral ischemia and discuss possible mechanisms usingmiddle cerebral artery occlusion model. Rats were randomly allocated to three groups: control, middle cerebral artery occlusion, and middle cerebral artery occlusion plus exercise. The middle cerebral artery occlusion plus exercise group was preconditioned by three weeks of voluntary wheel running prior to surgery. The accelerated rotarod test was employed to evaluate motor performance. Infarct volumes were analyzed to detect the neuroprotective effect of voluntary exercise. Brain-derived neurotrophic factor, Bax, Bcl-2, and caspase-3 protein expressions were measured by Western blot. Behavior evaluation showed the middle cerebral artery occlusion plus exercise group achieved significantly longer time on a rotarod than the unexercised group. Additionally, voluntary exercise reduced cerebral infarction and increased brain derived neurotrophic factor expression. Exercise down-regulated the apoptotic Bax/Bcl-2 ratio and caspase-3 protein expression. Results indicate that voluntary wheel running promote hippocampal brain derived neurotrophic factor and inhibit cell apoptosis in ischemia-induced impairment.

Hippocampal ischemia
voluntary exercise
brain-derived neurotrophic factor
Figure 1.
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