IMR Press / FBS / Volume 7 / Issue 1 / DOI: 10.2741/S421

Frontiers in Bioscience-Scholar (FBS) is published by IMR Press from Volume 13 Issue 1 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Object recognition test for studying cognitive impairments in animal models of Alzheimer’s disease
Show Less
1 Department of Pharmacology and Toxicology, University of Navarra, 31008 Pamplona, Spain

*Author to whom correspondence should be addressed.


Front. Biosci. (Schol Ed) 2015, 7(1), 10–29;
Published: 1 June 2015
(This article belongs to the Special Issue The polyhedral aspects of dementia)

Animal models are essential resources in basic research and drug discovery in the field of Alzheimer’s disease (AD). As the main clinical feature in AD is cognitive failure, the ultimate readout for any interventions or the ultimate goal in research should be measures of learning and memory. Although there is a wealth of genetic and biochemical studies on proposed AD pathogenic pathways, the aetiology of the illness remains unsolved. Therefore, assessment by cognitive assays should target relevant memory systems without assumptions about pathogenesis. The description of several tests that are available for assessing cognitive functioning in animal models can be found in literature. Among the behavioural test, the novel object recognition (NOR) task is a method to measure a specific form of recognition memory. It is based on the spontaneous behaviour of rodents and offers the advantage of not needing external motivation, reward or punishment. Therefore, the NOR test has been increasingly used as an experimental tool in assessing drug effects on memory and investigating the neural mechanisms underlying learning and memory. This review describes the basic procedure, modifications, practical considerations, and the requirements and caveats of this behavioural paradigm to be considered as appropriate for the study of AD. Altogether, NOR test could be considered as a very useful instrument that allows researchers to explore the cognitive status of rodents, and hence, for studying AD related pathological mechanisms or treatments.

Transgenic models
Sporadic Alzheimer’s disease
Amyloid beta
Back to top