IMR Press / FBS / Volume 5 / Issue 2 / DOI: 10.2741/S398

Frontiers in Bioscience-Scholar (FBS) is published by IMR Press from Volume 13 Issue 1 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Migration of retinal pigment epithelial cells is EGFR/PI3K/AKT dependent
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1 Department of Ophthalmology, Shanghai First People’s Hospital Affiliated Shanghai Jiao Tong University, 100 Haining road, Shanghai, PR China
2 Shanghai Eye Research Institute, 100 Haining road, Shanghai, PR China
3 School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan RD. Minhang District, Shanghai, PR China

*Author to whom correspondence should be addressed.

Front. Biosci. (Schol Ed) 2013, 5(2), 661–671;
Published: 1 January 2013

Abnormal migration of retinal pigment epithelium (RPE) contributes to a variety of disorders such as proliferative vitreoretinopathy. Here, the effect of epidermal growth factor (EGF), and signaling by its receptor (ERGR)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) on RPE cell migration was studied. The in vitro wound healing and migration of the human RPE cell line, ARPE19 cell was accelerated, in a dose dependent manner, in response to EGF stimulation, while pretreatment with EGFR, PI3K or AKT inhibitor, inhibited both events. Exposure of cells to EGF activated the AKT phosphorylation, whereas EGFR and PI3K inhibitors blocked EGFinduced AKT phosphorylation in a dose-dependent manner. These data suggest that EGF mediate ARPE-19 cell migration through EGFR/PI3K/AKT signaling pathway.

ARPE-19 cell
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