IMR Press / FBS / Volume 5 / Issue 1 / DOI: 10.2741/S361

Frontiers in Bioscience-Scholar (FBS) is published by IMR Press from Volume 13 Issue 1 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


Current status of human pluripotent stem cell based in vitro toxicity tests

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1 Systems toxicology unit, ECVAM, European Centre for the Validation of Alternative Methods, T.P. 580, IHCP, JRC, European Commission, via E. Fermi 2749, 21027 Ispra,VA, Italy
2 Institute of Neurophysiology, University of Cologne, Robert-Koch- Strasse 39, D-50931 Cologne, Germany
Academic Editor:Agapios Sachinidis
Front. Biosci. (Schol Ed) 2013, 5(1), 118–133;
Published: 1 January 2013

The present review assesses the current status of in vitro tests based on human pluripotent stem cell-derived toxicologically relevant target cells. The majority of the evaluated test systems are in the phase of test development. In particular the success rates of differentiation protocols and their reproducibility are varying depending on different culture conditions but also on the assessed marker panel and the functional evaluation of the cells. However, the amount of differentiated cells decreases in relation to their maturation status. No harmonization has been achieved yet about the required maturation status of the cellular models to be used for toxicological applications. Even with an established cellular model, the selection of appropriate readouts is challenging. Some areas of toxicity, such as developmental toxicity, suffer from insufficient knowledge on predictive biomarkers which leads to difficulties in the selection of the most appropriate endpoints. In this heterogeneous context the rapidly increasing knowledge about ‘omics’ technologies, might lead to an improvement of the current situation and allow the establishment of more predictive human in vitro toxicity tests.

Human pluripotent stem cells
In vitro toxicity testing
Test development criteria
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