IMR Press / FBS / Volume 10 / Issue 2 / DOI: 10.2741/S516

Frontiers in Bioscience-Scholar (FBS) is published by IMR Press from Volume 13 Issue 1 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


Protective effect of Zizyphus spinachristi on MPP+-induced oxidative stress

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1 Dept of Food Science and Nutrition, College of Agriculture and Marine Sciences, Sultan Qaboos University, Oman
2 Ageing and Dementia Research Group, Sultan Qaboos University, Oman
3 Department of Biotechnology, School of Biosciences, Periyar University, Periyar Palgalai nagar, Salem-636011, Tamilnadu, India
4 Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, 608 002, Tamil Nadu, India
5 Department of Biotechnology, Bharathiyar Unviersity, Coimbatore, India

*Author to whom correspondence should be addressed.

Front. Biosci. (Schol Ed) 2018, 10(2), 285–299;
Published: 1 January 2018

Oxidative stress and mitochondrial dysfunction mediated neuro apoptosis is reported to play a major role in the pathology of Parkinson’s disease. Zizyphus spina-christi fruits (ZSCF) are used as traditional medicines that are well-known for their high antioxidant properties. In the present study, we investigated the protective effects of ZSCF extract against 1-methyl-4-phenylpyridinium (MPP+) induced neurotoxicity in SH-SY5Y cell lines. The effect of ZCSF on MPP+ induced cell viability (MTT - 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay), membrane damage - (lactate dehydrogenase (LDH), oxidative stress (levels of ROS, nitric oxide and GSH and activities of SOD and catalase), mitochondrial membrane potential and apoptosis (activity of caspase 3 and protein expressions of cyto c, Bax and Bcl-2) were measured. Our results showed that ZSCF could be able to reduce the neurotoxicity of MPPand offer neuroprotection in vitro. This protective effect of ZCF might be mediated by its potent antioxidant properties. However, further research is necessary to isolate active compounds and performing preclinical and clinical studies to confirm the neuro-protective effects of ZSCF in PD.

Parkinson’s disease
Zizyphusspina Christi Fruit Extract
SH-SY5Y cells
Oxidative Stress
Mitochondrial dysfunction
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