IMR Press / FBS / Volume 10 / Issue 2 / DOI: 10.2741/S513

Frontiers in Bioscience-Scholar (FBS) is published by IMR Press from Volume 13 Issue 1 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis

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1 Department of Biotechnology, School of Biosciences, Periyar University, Periyar Palgalai nagar, Salem-636011, Tamilnadu, India
2 Department of Biochemistry and Biotechnology, Annamalai University, Annamalai nagar-608 002, Tamilnadu, India
3 Department of Food Science and Nutrition, CAMS, Sultan Qaboos University, Muscat, Oman
4 Ageing and Dementia Research Group, Sultan Qaboos University, Muscat, Oman
5 Food and Brain Research Foundation, Chennai, Tamil Nadu 600094, India
6 NIAA, National Institute of Health, Rockville, MD, USA
7 Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School and Shriners Hospitals for Children, Boston, MA 02114, USA

*Author to whom correspondence should be addressed.

Front. Biosci. (Schol Ed) 2018, 10(2), 248–261;
Published: 1 January 2018

The present study was carried out to investigate the neuroprotective effects of isolongifolene (ILF), a tricyclic sesquiterpene of Murraya koenigii, against rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis in a cellular model. SH-SY5Y human neuroblastoma cells were divided into four experimental groups (control, rotenone (100 nM), ILF (10 microM) + rotenone (100 nanoM), ILF 10 microM alone treated) based on 3-(4, 5-dimethyl 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results of the present study showed that the ILF treatment significantly alleviated rotenone-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in SH-SY5Y cells. Moreover, ILF attenuated rotenone induced toxicity by down-regulating Bax, caspases-3, 6, 8 and 9 expression and up-regulating of Bcl-2 expression. Furthermore regulation of p-P13K, p-AKT and p-GSK-3 beta expression by ILF, clearly confirmed its protective effects. Taken together, our results suggested that ILF attenuated rotenone-induced oxidative stress, mitochondrial dysfunction and apoptosis through the regulation of P13K/AKT/GSK-3 beta signaling pathways. However further pre-clinical studies are warranted in rodents to use ILF as a promising therapeutic agent for PD in future.

Oxidative Stress
Mitochondrial Dysfunction
Signaling Pathway
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