IMR Press / FBL / Volume 9 / Issue 2 / DOI: 10.2741/1312

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Rapidity and multiplicity of synthesis and expression of immunoglobulin isotypes by B lymphocytes in the small intestine
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1 Biology Department, School of Natural Sciences, California State University, 5500 University Parkway, San Bernardino, California 92407
2 Biology Program, California State University Channel Islands, One University Drive, Camarillo, California 93012
Front. Biosci. (Landmark Ed) 2004, 9(2), 1642–1653; https://doi.org/10.2741/1312
Published: 1 May 2004
Abstract

An immunofluorescence double labeling assay was used to examine the kinetics of intestinal B lymphocytes with concurrent expression of multiple antibody isotypes in the mucosal tissues of rats infected with Trichinella spiralis (TS) muscle larvae for 1 to 15 days. As compared to the uninfected controls (day 0), the non-Peyer's patch tissues of the small intestine contained a significantly increased number of dual antibody-expressing B cells as early as 3 days after infection with a maximum proliferation of these B cells on days 7 and 10. These results indicate the rapidity of B cell response in the small intestine. Similar results were observed in the germinal centers of the Peyer's patches. The non-germinal centers of the Peyer's patch tissues showed delayed kinetics in B cell activation which occurred 10 days after infection. Quantification of the total number of B cells in these tissues was also carried out by staining the CD45RA marker on B cells with the OX33 monoclonal antibody. When comparing the total numbers of B cells with the numbers of B cells expressing dual isotypes of antibodies, our results showed the numbers of dual-expressing B cells (IgA:IgE, IgM:IgE, IgG1:IgE, IgG2a:IgE, IgG2b:IgE, and IgG2c:IgE), when combined, were over 7 times that of the total number of OX33-labeled B cells on day 7 in the small intestine. The dual-expressing B cells in the Peyer's patch-germinal center were more than 5 times that of the OX33-labeled B cells on day 15. These results therefore suggest that the dual-expressing B cells most likely synthesized and expressed more than two isotypes of antibodies during the peak days of the humoral response. Such phenomenon was not observed in non-germinal centers of the Peyer's patch tissues.

Keywords
Trichinella spiralis
B lymphocytes
B cells
Antibody
Antigen
fluorescein isothiocyanate
rhodamine isothiocyanate
OX33
CD45RA
Small intestine
Peyer’s Patch
Germinal Center
Villus crypt
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