IMR Press / FBL / Volume 5 / Issue 3 / DOI: 10.2741/sczakiel

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Article
Theoretical and experimental approaches to design effective antisense oligonucleotides
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1 Forschungsschwerpunkt Angewandte Tumorvirologie-F0200, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany
Academic Editor:Eric Wickstrom
Front. Biosci. (Landmark Ed) 2000, 5(3), 194–201; https://doi.org/10.2741/sczakiel
Published: 1 January 2000
(This article belongs to the Special Issue Anti-sense therapeutics)
Abstract

Among the large number of possible antisense oligonucleotides (asODN) against a given target nucleic acid, only a small number of species seems to give rise to satisfactorily strong inhibition of target gene expression in living cells. Therefore much attention is paid to strategies that help to successfully design effective asODN. Here, selected experimental approaches and theoretical concepts will be briefly described that have been developed to increase the probability of success in the use of asODN. Advantages and disadvantages of these strategies will be compared and the relatively new and controversially discussed concept of a theoretical and computer-supported design of effective asODN will be addressed.

Keywords
asODN
asODN
design
asODN
Annealing
bcrabl
HIV-1
Kinetic probing
RNA
Structure
Review
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