IMR Press / FBL / Volume 5 / Issue 3 / DOI: 10.2741/furlow

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Proteasomes in the archaea: from structure to function
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1 Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611-0700, USA
Academic Editor:Everly Conway de Macario
Front. Biosci. (Landmark Ed) 2000, 5(3), 837–865; https://doi.org/10.2741/furlow
Published: 1 September 2000
Abstract

Survival of cells is critically dependent on their ability to rapidly adapt to changes in the natural environment no matter how 'extreme' the habitat. An interplay between protein folding and hydrolysis is emerging as a central mechanism for stress survival and proper cell function. In eucaryotic cells, most proteins destined for destruction are covalently modified by the ubiquitin-system and then degraded in an energy-dependent mechanism by the 26S proteasome, a multicatalytic protease. The 26S proteasome is composed of a 20S proteolytic core and 19S cap (PA700) regulator which includes six AAA+ ATPase subunits. Related AAA+ proteins and 20S proteasomes are found in the archaea and Gram positive actinomycetes. In general, 20S proteasomes form a barrel-shaped nanocompartment with narrow openings which isolate rather non-specific proteolytic active-sites to the interior of the cylinder and away from interaction with cytosolic proteins. The proteasome-associated AAA+ proteins are predicted to form ring-like structures which unfold substrate proteins for entry into the central proteolytic 20S chamber resulting in an energy-dependent and processive destruction of the protein. Detailed biochemical and biophysical analysis as well as identification of proteasomes in archaea with developed genetic tools are providing a foundation for understanding the biological role of the proteasome in these unusual organisms.

Keywords
Archaea
Proteasome
PAN
AAA ATPase
HslV
Protease
Chaperones
Stress Response
Review
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