IMR Press / FBL / Volume 4 / Issue 4 / DOI: 10.2741/macpherson

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Ribozymes in gene therapy of HIV-1
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1 Johnson & Johnson Research Laboratories, GPO Box 3331, Sydney, NSW 2001, Australia
Academic Editors:Joseph Rosenblatt, Vicente Planelles
Front. Biosci. (Landmark Ed) 1999, 4(4), 497–505;
Published: 1 June 1999
(This article belongs to the Special Issue Gene therapy approaches to HIV-1 Infection)

Human immunodeficiency virus type 1 (HIV-1) is the primary etiologic agent for Aquired Immune Deficiency Syndrome (AIDS). HIV-1 is a lentivirus, a separate genus of the Retroviridae, which are complex RNA viruses that integrate into the genome of host cells and replicate intracellularly. Ribozymes are catalytic RNA molecules with enzyme-like cleavage properties, that can be designed to target specific RNA sequences within the HIV-1 genome. In addition to the genomic RNA, several RNA intermediates, including splice variants, can be targeted by a single ribozyme. We and others have demonstrated the ability of ribozymes to suppress HIV-1 replication in a variety of cultured cells. Ribozyme gene therapy for HIV-1 infection is a therapeutic approach that offers several potential advantages over conventional therapies in that it can potentially impact on both viral load and restoration of the immune system. Ribozyme gene therapy may be used as an adjunct to chemotherapeutic drugs, effecting viral suppression, and facilitating immune restoration without problems of patient compliance. Currently, an anti-HIV-1 ribozyme is being tested in two separate Phase I Clinical Trials.

Gene therapy
CD4+ lymphocytes
CD34+ stem cells
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