IMR Press / FBL / Volume 26 / Issue 6 / DOI: 10.52586/4928
Open Access Original Research
Acetylsalicylic acid improves cognitive performance in sleep deprived adult Zebrafish (Danio rerio) model
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1 Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education & Research, 570015 Mysuru, India
2 Centre for Experimental Pharmacology & Toxicology, Central Animal Facility, JSS Academy of Higher Education & Research, 570015 Mysuru, India
3 Department of Processing Technology (Biochemistry), Kerala University of Fisheries and Ocean Studies, Panangad, 682506 Kochi, Kerala, India
4 Regional Research and Educational Centre, Tamil Nadu Veterinary and Animal Sciences University, 622004 Pudukkottai, India
5 Biodiversity Research Centre, Dohfar University, 211 Salalah, Sultanate of Oman
6 College of Pharmacy and Nutrition, University of Saskatchewan, 107, Wiggins Road, Saskatoon, SK S7N 5C9, Canada
7 Department of Biological Sciences and Institute of Neuroimmune Pharmacology, Seton Hall University, 400 South Orange Ave South Orange, NJ 07079, USA
8 Department of Food Science and Nutrition, CAMS, Sultan Qaboos University, 123 Muscat, Oman
9 Ageing and Dementia Research Group, Sultan Qaboos University, 123 Muscat, Oman
*Correspondence: (Meena Kishore Sakharkar); (Saravana Babu Chidambaram)
Front. Biosci. (Landmark Ed) 2021, 26(6), 114–124;
Submitted: 3 January 2021 | Accepted: 15 February 2021 | Published: 30 May 2021
Copyright: © 2021 The Author(s). Published by BRI.
This is an open access article under the CC BY 4.0 license (

Sleep deprivation (SD) is commonly associated with decreased attention, reduced responsiveness to external stimuli, and impaired locomotor and cognitive performances. Strong evidence indicates that SD disrupts neuro-immuno-endocrine system which is also linked to cognitive function. Recently Zebrafish have emerged as a powerful model sharing organizational and functional characteristics with other vertebrates, providing great translational relevance with rapid and reliable screening results. In the current study, we examined the effects of acetylsalicylic acid (aspirin) on cognitive and locomotor activity in sleep deprived Zebrafish model. Learning and memory were assessed by T-maze and locomotor activity was assessed by partition preference and swimming time in spinning tasks. Furthermore, brain bioavailability of aspirin was determined by high performance liquid chromatography. Following drug exposure and tasks, histopathology of the brain was performed. It was observed that three-day SD significantly reduces learning and memory and locomotion in the Zebrafish. Aspirin was found to restore SD induced cognitive decline and improve the locomotor functions. Neuro-inflammation and impaired functional network connectivity is linked to cognitive defects, which implicate the possible benefits of immunotherapeutics. In the present study, aspirin decreased neutrophil infiltration, and increased spine density in dentate gyrus granular and shrinkage and basophil in the CA1 neurons of hippocampus. This hints the benefit of aspirin on neuroimmune functions in sleep deprived fish and warrants more studies to establish the clear molecular mechanism behind this protective effect.

Sleep deprivation
Acetylsalicylic acid
Spinning task
Fig. 1.
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