IMR Press / FBL / Volume 24 / Issue 3 / DOI: 10.2741/4729

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Cytokine gene expression following RSV-A infection

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1 Cell and Developmental Biology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
2 Disease Genetics and Biotechnology Lab, CARI, Izatnagar, UP-243 122, India
3 Tumor Immunology Lab, IVRI, Izatnagar, UP-243 122, India
4 Animal Science Department, Rohilkhand University, Bareilly (UP), India
5 Department of Pharmaceutical Sciences, St. John’s University, Queens, USA
6 Department of Pharmacology and Experimental Therapeutics, The University of Toledo, Toledo, OH 43614, USA

*Author to whom correspondence should be addressed.

Front. Biosci. (Landmark Ed) 2019, 24(3), 463–481; https://doi.org/10.2741/4729
Published: 1 January 2019
Abstract

The present study determines the cytokine gene expression in chickens following RSV-A infection, using RT-qPCR. In susceptible chickens tumors progressed to fulminating metastatic tumors while it regressed in regressors chickens and some resistant non-responder chickens did not respond to RSV-A infection and thus did not develop tumors at all. The in vivo expression of pro-inflammatory cytokines, Th1 cytokines and Th2 cytokines was determined at the primary site of infection, as well as in different organs of progressor, regressor and non-responder chicks at different time intervals. Our results indicated a significant upregulation of the pro-inflammatory cytokines, IL-6 and IL-8, in all the organs of progressor chicks, while they were significantly lower in regressor and non-responder chicks. The expression of the Th1 cytokines IFN-γ and TNF-α was low in all of the organs of the progressor group, except that in spleen. In contrast, regressor and non-responder groups showed high expression of IFN-γ and TNF-α. Further, there was an early upregulation of the expression of the Th2 cytokine, IL-10, TGF-β and GM-CSF, in all of the organs of progressors as compared to uninfected control.

Keywords
Cytokine
Gene expression
RSV-A
chickens
Tumor
mRNA
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