IMR Press / FBL / Volume 23 / Issue 4 / DOI: 10.2741/4607

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


Mass spectrometry-based proteomic analysis of the DNA damage response

Show Less
1 Cell Signaling Technology, INC. 3 Trask Lane, Danvers, MA 01923, USA
Front. Biosci. (Landmark Ed) 2018, 23(4), 597–613;
Published: 1 January 2018
(This article belongs to the Special Issue DNA damage, DNA repair, and DNA damage response pathways)

In response to DNA damage, cells have evolved mechanisms to halt cell cycle progression, activate repair, or initiate apoptosis. These DNA damage response (DDR) pathways are critical for cellular survival in response to genomic insult, and play important roles in growth, development, and disease. Historically, mediators of DNA damage response signaling have been studied one or a few proteins at a time. Advances in mass spectrometry instrumentation and enrichment methods now allow for more global analysis of the DDR in cells and tissues. In this review we will discuss current methods in liquid chromatography tandem mass spectrometry (LC-MS/MS), enrichment strategies, and targeted analyses for the study of damage signaling. These methods have allowed a greater understanding of the DNA damage response and have highlighted the far-reaching effects of activation of damage-induced pathways.

DNA Damage
Signaling Pathway
Back to top