IMR Press / FBL / Volume 23 / Issue 3 / DOI: 10.2741/4601

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


DNA replication machinery is required for development in Drosophila

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1 Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto, Japan
2 Department of Cell Biology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto, Japan 606-8507
3 Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
4 Department of Molecular Cellular and Biochemistry, The Ohio State Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA
Front. Biosci. (Landmark Ed) 2018, 23(3), 493–505;
Published: 1 January 2018

In Drosophila, some factors involved in chromosome replication seem to be involved in gene amplification and endoreplication, which are actively utilized in particular tissue development, but direct evidence has not been shown. Therefore, we examined the effect of depletion of replication factors on these processes. First, we confirmed RNAi knockdown can be used for the depletion of replication factors by comparing the phenotypes of RNAi knockdown and deletion or point mutants of the components of DNA licensing factor, MCM2, MCM4 and Cdt1. Next, we found that tissue-specific RNAi knockdown of replication factors caused tissue-specific defects, probably due to defects in DNA replication. In particular, we found that depletion inhibited gene amplification of the chorion gene in follicle cells and endoreplication in salivary glands, showing that chromosomal DNA replication factors are required for these processes. Finally, using RNAi, we screened the genes for chromosomal DNA replication that affected tissue development. Interestingly, wing specific knockdown of Mcm10 induced wing formation defects. These results suggest that some components of chromosomal replication machinery are directly involved in tissue development.

DNA replication
Tissue specific RNAi-knockwown
Gene Amplification
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