IMR Press / FBL / Volume 21 / Issue 5 / DOI: 10.2741/4434

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


Cell type-specific transcriptome profiling in mammalian brains

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1 Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, One Lomb Memorial Dr., Rochester, NY 14623, USA
Academic Editor:Feng Cui
Front. Biosci. (Landmark Ed) 2016, 21(5), 973–985;
Published: 1 June 2016
(This article belongs to the Special Issue Transcriptome of mammalian brain)

A mammalian brain contains numerous types of cells. Advances in neuroscience in the past decade allow us to identify and isolate neural cells of interest from mammalian brains. Recent developments in high-throughput technologies, such as microarrays and next-generation sequencing (NGS), provide detailed information on gene expression in pooled cells on a genomic scale. As a result, many novel genes have been found critical in cell type-specific transcriptional regulation. These differentially expressed genes can be used as molecular signatures, unique to a particular class of neural cells. Use of this gene expression-based approach can further differentiate neural cell types into subtypes, potentially linking some of them with neurological diseases. In this article, experimental techniques used to purify neural cells are described, followed by a review on recent microarray-or NGS-based transcriptomic studies of common neural cell types. The future prospects of cell type-specific research are also discussed.

Cell Type-Specific
Next-Generation Sequencing (NGS)
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