IMR Press / FBL / Volume 21 / Issue 4 / DOI: 10.2741/4415

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Regulation of dynein-mediated autophagosomes trafficking by ASM in CASMCs

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1 Department of Pharmacology and Toxicology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA
2 Laboratory of Chinese Herbal Pharmacy, Renmin Hospital, Hubei University of Medicine, Shiyan, 442000, China
3 Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, TX 77204, USA
Academic Editor:Pin-Lan Li
Front. Biosci. (Landmark Ed) 2016, 21(4), 696–706; https://doi.org/10.2741/4415
Published: 1 January 2016
(This article belongs to the Special Issue Molecular pathobiology of lipid mediators)
Abstract

Acid sphingomyelinase (ASM; gene symbol Smpd1) has been shown to play a crucial role in autophagy maturation by controlling lysosomal fusion with autophagosomes in coronary arterial smooth muscle cells (CASMCs). However, the underlying molecular mechanism by which ASM controls autophagolysosomal fusion remains unknown. In primary cultured CASMCs, lysosomal Ca2+ induced by 7-ketocholesterol (7-Ket, an atherogenic stimulus and autophagy inducer) was markedly attenuated by ASM deficiency or TRPML1 gene silencing suggesting that ASM signaling is required for TRPML1 channel activity and subsequent lysosomal Ca2+ release. In these CASMCs, ASM deficiency or TRPML1 gene silencing markedly inhibited 7-Ket-induced dynein activation. In addition, 7-Ket-induced autophagosome trafficking, an event associated with lysosomal Ca2+ release and dynein activity, was significantly inhibited in ASM-deficient (Smpd1-/-) CASMCs compared to that in Smpd1+/+ CASMCs. Finally, overexpression of TRPML1 proteins restored 7-Ket-induced lysosomal Ca2+ release and autophagosome trafficking in Smpd1-/- CASMCs. Collectively, these results suggest that ASM plays a critical role in regulating lysosomal TRPML1-Ca2+ signaling and subsequent dynein-mediated autophagosome trafficking, which leads its role in controlling autophagy maturation in CASMCs under atherogenic stimulation.

Keywords
Smooth Muscle Cells
Autophagy Maturation
Sphingomyelinase
Lysosomal Ca2+
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