IMR Press / FBL / Volume 21 / Issue 2 / DOI: 10.2741/4400

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Cry1Ab/c in different stages of growth in transgenic rice Bt-shanyou63

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1 State Key Laboratory of Biosafty, Nanjing Institute of Environmental Sciences (NIES), Ministry of Environmental Protection of China, No 8, Jiang-wang-miao St., Nanjing, Jiangsu 210042, China
2 College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095,China
Front. Biosci. (Landmark Ed) 2016, 21(2), 447–454; https://doi.org/10.2741/4400
Published: 1 January 2016
Abstract

The relationship between the mRNA level and the corresponding protein level of the cry1Ab/c gene is not well characterized in transgenic rice (Bt-ShanYou63). In this study, we compared cry1Ab/c mRNA and its protein expression in leaves at different growth stages in Bt-ShanYou63 rice. The results demonstrated that both cry1Ab/c mRNA and its protein levels changed at all of the growth stages. The cry1Ab/c transcript levels in the leaves were highest during the grain filling stage (3.29, cry/actin) and lowest during the seeding stage (1.06, cry/actin), and the protein levels of Cry1Ab/c was also highest at the grain filling stage (5.71 µg×g-1 fresh weight, fw) and lowest during the seeding stage (2.08 µg×g-1 fw). There was a significant correlation between cry1Ab/c mRNA levels and the protein concentrations (r=0.742, p < 0.01). However, a linear relationship was not observed between cry1Ab/c mRNA levels and the protein levels, and the trend for mRNA expression levels was not consistent with the Cry1Ab/c protein levels in the same growth period in Bt-ShanYou63 rice.

Keywords
Bt Rice
cry1Ab/c mRNA
Cry1Ab/c Protein
Correlation
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