IMR Press / FBL / Volume 20 / Issue 7 / DOI: 10.2741/4357

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Expression of AQP3 protein in hAECs is regulated by Camp-PKA-CREB signalling pathway
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1 Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China
Academic Editor:Fei He
Front. Biosci. (Landmark Ed) 2015, 20(7), 1047–1055; https://doi.org/10.2741/4357
Published: 1 June 2015
(This article belongs to the Special Issue Cellular immunology and stem cell biology)
Abstract

Previous studies by others and our group have demonstrated the expression of AQP3 protein in human chorioamniotic membranes. Here, we investigated whether cyclic adenosine monophosphate (cAMP) up-regulation of aquaporin 3 (AQP3) protein expression in human amniotic epithelial cells (hAECs) is mediated by protein kinase A (PKA) dependent or independent pathway. Cells were treated with various concentrations of Forskolin, SP-cAMP, H-89 at various time intervals or optimal concentration of Forskolin in combination with H-89 in the blocking experiments. Forskolin significantly increased cAMP levels and the expression of PKA, p-CREB and AQP3. SP-cAMP had similar effects. H-89 inhibited PKA, p-CREB and AQP3 protein expression, and attenuated the stimulatory effect of Forskolin. These results show that the AQP3 protein expression in hAECs was regulated by cAMP-PKA-CREB signal pathway. A relatively short biological half life of AQP3 renders its rapid responsiveness to regulation.

Keywords
Aquaporin 3
Cyclic Adenosine Monophosphate
Protein Kinase A
Placenta
Amniotic Epithelial Cells
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