IMR Press / FBL / Volume 20 / Issue 6 / DOI: 10.2741/4350

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Thymic derived iPs cells can be differentiated into cardiomyocytes
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1 Children’s Hospital of Fudan University, Shanghai 201102, China
2 Shanghai Key Laboratory of Birth Defects, Shanghai 201102, China
3 Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical College of Fudan University, Shanghai 200032, China
Academic Editor:Xu Jin
Front. Biosci. (Landmark Ed) 2015, 20(6), 964–974; https://doi.org/10.2741/4350
Published: 1 June 2015
(This article belongs to the Special Issue The dual role of ERK signaling in the apoptosis of neurons)
Abstract

Ventricular septal defect (VSD) is a common congenital heart malformation. Several factors lead to the development of VSD, including familial causes, exposure to certain drugs, infectious agents, and maternal metabolic disturbances. We hypothesized that induced pluripotent stem (iPS) cells can be obtained from VSD patients to generate cardiomyocytes. Here, we show the generation and cardiomyocyte differentiation of iPS cells from the thymic epithelial cells of a patient with VSD (TECs-VSD) by overexpressing four transcription factors: OCT4, SOX2, NANOG, and LIN28 using lentiviral vectors. The self-renewal capacity and pluripotency of iPS cells was verified in vitro by expression of pluripotency markers and formation of teratoma in vivo. The results show that iPS cells can be derived from patients with VSD and they can be differentiated into cardiomyocytes. These cells can be used for understanding the pathogenesis of defect that causes VSD.

Keywords
Ventricular Septal Defect
Pluripotent Stem Cells
Differentiation
Cardiomyocyte
Malformations
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