IMR Press / FBL / Volume 13 / Issue 10 / DOI: 10.2741/2970

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


Genotypic variation of Candida albicans during orthodontic therapy

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1 Orthodontics, Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Hong Kong SAR
2 Oral Bio-Sciences, Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Hong Kong SAR

*Author to whom correspondence should be addressed.


Front. Biosci. (Landmark Ed) 2008, 13(10), 3814–3824;
Published: 1 May 2008

Candida is a common oral, opportunistic pathogen. The aim of this longitudinal study was to analyze the genotypes of sequential isolates of Candida albicans in a cohort of healthy consistent candidal carriers ('carriers') during fixed orthodontic appliance therapy. 11 of 97 subjects were 'carriers'. Candida isolates from baseline samples (T0) and sequential visits after insertion (T1 to T10) were speciated and RAPD fingerprint patterns of 101 sequential Candida albicans isolates were analyzed with PCR and gel electrophoresis. A similarity coefficient (SAB) for each pair of strains was calculated and clusters of similar strains grouped using Dendrogram analysis of the RAPD gel profiles. The composite dendrogram of all isolates indicated that the Candida populations in this cohort are genotypically dissimilar although collected from the same geographic locale and, from a similar healthy, age group. Genotypes of a majority of the cohort (6 of 11 consistent carriers) show minor evolutionary variations in profile and genotypic 'shuffling', implying this may be a survival mechanism of this common, human, opportunist pathogen residing in a hostile oral environment.

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