IMR Press / FBL / Volume 12 / Issue 6 / DOI: 10.2741/2224

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Direct AAV-mediated gene delivery to the temporomandibular joint
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1 The Biomedical and Tissue Engineering Group, Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, SAR, Hong Kong
Front. Biosci. (Landmark Ed) 2007, 12(6), 2212–2220; https://doi.org/10.2741/2224
Published: 1 January 2007
Abstract

Successful intra-temporomandibular joint gene transfer is a prerequisite for gene therapy to induce mandibular condylar growth. This study was carried out to investigate the expression of recombinant adeno-associated virus (rAAV) based vector-mediated enhanced green fluorescence protein (eGFP) in the mandibular condyle. The transduction efficiencies for primary chondrocytes using different hybrids of rAAV vectors were identified by fluorescence microscopy and FACS. Sprague-Dawley rats were injected with either rAAV2/2-eGFP construct or saline into both mandibular condyles. The spatial and temporal transgene expression was detected by in situ hybridization, RT-PCR and real-time PCR. Our result showed that rAAVs were capable of infecting rat chondrocytes. rAAV2/2 was the best serotype to infect chondrocytes in vitro. By in situ hybridization, eGFP expression was clearly detected in the deeper layer of mandibular condyle as early as 7 days after injection. By real-time PCR, the transgene expression reached a peak at 21 days. This study was the first report to explore that rAAV-mediated genes could be transferred to mandibular condyle in vivo. Our results strongly suggest that rAAV2/2 mediated gene delivery is a promising approach to deliver candidate genes for future regulating mandibular condylar growth.

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