IMR Press / FBL / Volume 11 / Issue 3 / DOI: 10.2741/2037

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

MMP-9 expression is associated with leukocytic but not endothelial markers in brain arteriovenous malformations
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1 Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA 94110, USA
2 Departments of Neurological Surgery, University of California, San Francisco, CA 94110
3 Departments of Neurology, University of California, San Francisco, CA 94110
4 Departments of Epidemiology and Biostatistics, University of California, San Francisco, CA 94110
5 Departments of Pathology, University of California, San Francisco, CA 94110
Academic Editor:Yongmei Chen
Front. Biosci. (Landmark Ed) 2006, 11(3), 3121–3128;
Published: 1 September 2006
(This article belongs to the Special Issue Mechanisms of stroke injury)

Brain arteriovenous malformations (BAVM) have high matrix metalloproteinase-9 (MMP-9) expression, the source of which is unclear. We hypothesized MMP-9 production might be due to inflammation in BAVM. Compared to control brain tissues (n=5), BAVM tissue (n=139) had a higher expression (by ELISA) of myeloperoxidase (MPO) (193±189 vs. 6±3, ng/mg, P<.001), MMP-9 (28±32 vs. 0.7±0.6, ng/mg, P<.001), and IL-6 (102±218 vs. 0.1±0.1, pg/mg, P<.001), but not eNOS (114±87 vs. 65±9, pg/mg, P=.09). MMP-9 expression in BAVM highly correlated with myeloperoxidase (R2=.76, P<.001), as well as with IL-6 (R2=.32, P<.001). In contrast, MMP-9 in BAVM poorly correlated with the endothelial marker, eNOS (R2=.03, P=.05), and CD31 (R2 = .004, P=.57). Compared to non-embolized patients (n=46), patients with pre-operative embolization (n=93) had higher levels of myeloperoxidase (236±205 vs. 106±108, ng/mg, P<.001) and MMP-9 (33±35 vs. 16±20, ng/mg, P<.001), however the correlation between MMP-9 and myeloperoxidase was equally strong for both groups (R2=.69, n=93, P<.001, for both). MMP-9 expression correlated with the lipocalin-MMP-9 complex, suggesting neutrophils as the MMP-9 source. MPO co-localized with majority of MMP-9 signal by immunohistochemistry. Our data suggest that inflammation is a prominent feature of BAVM lesional phenotype, and neutrophils appear to be a major source of MMP-9 in these lesions.

Matrix metalloprotease-9
Vascular Malformations
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