IMR Press / FBL / Volume 11 / Issue 2 / DOI: 10.2741/1884

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Identification and differentiation of hepatic stem cells during liver development
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1 Laboratory of Stem Cell Therapy, The Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
2 Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892

Academic Editor: Sherif Karam

Front. Biosci. (Landmark Ed) 2006, 11(2), 1302–1310; https://doi.org/10.2741/1884
Published: 1 May 2006
(This article belongs to the Special Issue Adult stem cell biology)
Abstract

Stem cells responsible for maintenance and repair of tissues are found in a number of organs. The liver's remarkable capacity to regenerate after hepatectomy or chemical-induced injury does not involve proliferation of stem cells. However, recent studies suggest that liver stem cells exist in both embryonic and adult livers. Using fluorescence-activated cell sorting and a culture system in which primitive hepatic progenitor cells form colonies, a novel class of cells with the marker profile c-Met+CD49f+/lowc-Kit(-)CD45-TER119- was found in the developing liver. This class apparently represents the population of cells that form colonies containing distinct hepatocytes and cholangiocytes. When cells in this class are transplanted into the spleen or liver of mice subjected to liver injury, the cells migrate and differentiate into liver parenchymal cells and cholangiocytes that are morphologically and functionally indistinguishable from their native counterparts. During mid-gestation, hematopoietic cells migrate into the liver from a region bounded by aorta, gonad, and mesonephros and produce oncostatin M (OSM). In combination with glucocorticoid hormones, OSM induces maturation of liver stem and progenitor cells, including those of the c-Met+CD49f+/lowc-Kit-CD45-TER119- class. The ability to manipulate the proliferation and differentiation of liver stem cells in vitro will greatly aid in analyzing mechanisms of liver development and offers promise in stem cell therapy of liver diseases.

Keywords
Liver
Stem Cell
Oval Cell
Oncostatin M
Hepatocyte Nuclear Factor
Extracellular Matrix
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