IMR Press / FBL / Volume 10 / Issue 3 / DOI: 10.2741/1740

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Fabrication of a hydrogen peroxide biosensor based on a self-assemble composite oxide film
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1 Key Lab of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing, 210093, China
Front. Biosci. (Landmark Ed) 2005, 10(3), 2841–2847; https://doi.org/10.2741/1740
Published: 1 September 2005
Abstract

A titania film with horseradish peroxidase (HRP) was immobilized on indium tin oxide (ITO) electrodes to act as a biosensor of hydrogen peroxide via the self-assembling and sol-gel route. The film was characterized by the atomic functional microscopy, electrochemical and UV-vis spectroscopy methods. The resulting biosensor exhibited fast amperometric response and good stability to hydrogen peroxide (H2O2). The linear range for H2O2 determination was from 2×10-6 to 1.45×10-3 M, with a detection limit of 4×10-6 M based on S/N=3. The apparent Michaelis-Menten constant of the biosensor to H2O2 was estimated to be 1.5 mM, showing the HRP on the films had the higher biologic activities.

Keywords
Titania
Biosensor
Sol-gel
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