IMR Press / FBL / Volume 10 / Issue 2 / DOI: 10.2741/1675

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
A reagentless biosensor of nitric oxide based on direct electron transfer process of cytochrome c on multi-walled carbon nanotube
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1 Anhui Key Laboratory of Functional molecular Solids, School of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China
Front. Biosci. (Landmark Ed) 2005, 10(2), 2005–2010; https://doi.org/10.2741/1675
Published: 1 September 2005
Abstract

Direct electron transfer between Cytochrome c (Cyt.c) and electrode can be achieved through immobilizing Cyt.c on the surface of multi-walled carbon nanotubes (MWNTs). Under the condition of cyclic potential scans, Cyt.c can be adsorbed on the surface of MWNTs that were modified on a glassy carbon (GC) electrode to form an approximate monolayer. The redox characteristic and bioactivity of Cyt.c could be remained after it was adsorbed on MWNTs' surface. This provides a way to construct a new biosenser based on the activity of Cyt.c. Further investigation displayed that Cyt.c adsorbed on MWNTs showed an enzyme-like activity to catalyze the reduction of nitric oxide (NO). Due to catalyzing by Cyt.c, the reduction of NO in aqueous solution was achieved, which reductive potential appeared at -0.747V (vs. SCE). The peak currents were linearly proportional to concentration of NO in the range from 2 to 48 micromol/l with a limit of detection of 1.3 µM. The biosensor showed a good stability and excellent repeatability.

Keywords
Cytochrome c
multi-walled carbon nanotube
direct electron- transfer
biosensor
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