IMR Press / FBL / Volume 10 / Issue 1 / DOI: 10.2741/1589

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Tissue-distribution of aldehyde dehydrogenase 2 and effects of the ALDH2 gene-disruption on the expression of enzymes involved in alcohol metabolism

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1 Department of Environmental Health, University of Occupational and Environmental Health, Kitakyushu, 807-8555, Japan
2 Department of Surgery II, University of Occupational and Environmental Health, Kitakyushu, 807- 8555, Japan
3 Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA
4 Department of Preventive Medicine, College of Medicine, Chungbuk National University, 48 San Kaeshin-dong, Hungdok-gu, Cheongju, Chungbuk 361-763, South Korea
5 Purdue University, Department of Biochemistry, 175 S. University Street, West Lafayette IN 47907-2063, USA

Academic Editor: Norio Kagawa

Front. Biosci. (Landmark Ed) 2005, 10(1), 951–960; https://doi.org/10.2741/1589
Published: 1 January 2005
(This article belongs to the Special Issue Gene regulation and structure-function of P450 Cold stress response)
Abstract

In alcohol metabolism, acetaldehyde, a highly reactive intermediate that may cause cellular and DNA damages, is converted to acetate by mitochondrial aldehyde dehydrogenase ALDH2. Although the majority of ingested alcohol is eliminated in the liver, the first-pass metabolism of ethanol in the upper digestive tract is also important for prevention and management of ethanol-related gastrointestinal diseases. However, the tissue-distribution of Aldh2 in mice has been poorly investigated. In this study, therefore, we investigated the tissue-distribution of Aldh2 as well as Aldh1, Cyp1a1, Cyp2e1, and Cyp4b1 in wild type and Aldh2-null mice by immuno-histochemical analysis. The human liver and esophageal tissues were also examined. In mice, the Aldh2 protein was detected in the liver, lung, heart, kidney, testis, esophagus, stomach, colon, and pancreas, suggesting that the tissue-distribution of Aldh2 in mice is similar to that in humans. Therefore, Aldh2-null mice may be useful model animals for the investigation of alcohol metabolism and related diseases. Compared with the wild type, the expression level of Cyp2e1 was increased in the liver from Aldh2-null mice based on Western blot analysis, whereas the levels of Aldh1, Cyp1a1, and Cyp4b1 were indistinguishable. This observation suggests that a metabolite(s) of Aldh2 might down-regulate the expression of Cyp2e1 gene.

Keywords
ALDH2
CYP2E1
Esophageal Cancer
Acetaldehyde Dehydrogenase
Alcohol metabolism
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