IMR Press / FBE / Volume 8 / Issue 1 / DOI: 10.2741/E753

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

GMP-grade platelet lysate enhances proliferation and migration of tenon fibroblasts

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1 Department of Medical-Surgical Science and Biotechnologies, Faculty of Pharmacy and Medicine, University of Rome, Sapienza, della Repubblica 79, 04100 Latina, Italy
2 Center for Life Nano Science, Sapienza, Istituto Italiano di Tecnologia, Rome, Italy
3 Department of Internal Medicine and Medical Specialties, University of Rome, Sapienza, Rome, Italy
4 Department of Ophthalmology, Sapienza University of Rome, Polo Pontino, A. Fiorini Hospital, Via Firenze, 04019 Terracina
5 Immunohematology and Transfusion Medicine, San Camillo Forlanini Hospital, Rome, Italy
6 Department of Experimental Medicine, University of Rome, Sapienza, Rome, Italy
7 IRCCS Neuromed, Località Camerelle, 86077 Pozzilli, Italy

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2016, 8(1), 84–99;
Published: 1 January 2016

Tenon’s fibroblasts (TFs), widely employed as in vitro model for many ophthalmological studies, are routinely cultured with FBS. Platelet Lysate (PL), a hemoderivate enriched with growth factors and cytokines has been largely tested in several clinical applications and as substitute of FBS in culture. Here, we investigate whether PL can exert biological effects on TF populations similarly to other cell types. Results show that PL significantly enhances cell proliferation and migration vs. FBS, without influencing cell size/granularity. Upregulation of EGF, VEGF, KDR, MMP2-9, FAK mRNA levels also occurs and phosphorylation of AKT but not of ERK1/2 is significantly enhanced. The inhibition of the PI3kinase/AKT pathway with the specific inhibitor wortmannin, decreases PL-induced cell migration but not proliferation. Condition supernatants containing PL show increased bioavailability of Nitric Oxide and reduced levels of 8-Iso-PGF2-alpha, correlating with cell proliferation and migration. Pro-angiogenic/inflammatory soluble factors (GRO, Angiogenin, EGF, I-309, PARC) are exclusively or greater expressed in media containing PL than FBS. GMP-grade PL preparations positively influence in vitro biological effects of TFs representing a suitable and safer alternative to FBS.

Tenon Fibroblasts
Platelet Lysate
Growth Factors
Oxidative Stress
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