IMR Press / FBE / Volume 6 / Issue 1 / DOI: 10.2741/E691

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Reproducibility of measuring cerebral blood flow by laser-Doppler flowmetry in mice
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1 Biophysics Program, Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan
2 Department of Neurological Surgery, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuoku, Chiba 260-8670, Japan
3 Center for Frontier Science and Engineering, University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan

*Author to whom correspondence should be addressed.

Academic Editor: Tetsuya Matsuura

Front. Biosci. (Elite Ed) 2014, 6(1), 62–68;
Published: 1 January 2014
(This article belongs to the Special Issue Brain activation and cerebral blood flow regulation)

Laser-Doppler flowmetry has been widely used to trace hemodynamic changes in experimental stroke research. The purpose of the present study was to evaluate the day-to-day test-retest reproducibility of measuring cerebral blood flow by LDF in awake mice. The flux indicating cerebral blood flow (CBF), red blood cell (RBC) velocity, and RBC concentration were measured with LDF via cranial windows for the bilateral somatosensory cortex in awake mice. LDF measurements were performed three times, at baseline, 1 hour after, and 7 days after the baseline measurement. Moreover, breathing rate (BR) and partial pressure of transcutaneous CO₂ (PtCO₂) were measured simultaneously with LDF measurement. Intraclass correlation coefficient (ICC) and within-subject coefficient of variation (CVw) were calculated. CBF, RBC velocity, and RBC concentration showed good day-to-day test-retest reproducibility (ICC: 0.61 - 0.95, CVw: 8.3% - 15.4%). BR and PtCO₂ in awake mice were stable during the course of the experiments. The evaluation of cerebral microcirculation using LDF appears to be applicable to long-term studies.

Laser-Doppler Flowmetry
Cerebral Blood Flow
Test-retest Reproducibility
Intraclass Correlation Coefficient
Within-subject Coefficient of Variation
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