IMR Press / FBE / Volume 5 / Issue 2 / DOI: 10.2741/E656

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Gap junction -mediated cAMP movement between oocytes and somatic cells

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1 State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2013, 5(2), 755–767; https://doi.org/10.2741/E656
Published: 1 January 2013
Abstract

Cyclic AMP (cAMP) plays a critical role in oocyte meiotic maturation. However, the source of cAMP surge prior to maturation and the direction of gap junction-dependent cAMP movement are unclear. In this study, inhibition of gap junctional communication (GJC) using carbenoxolone (3.5 h) induced meiotic resumption in ~90% of follicle-enclosed oocytes (FEOs). The concentration of cAMP in a single oocyte was higher than that in a single cumulus cell, suggesting that the movement of cAMP proceeds from the oocyte to cumulus cells under passive diffusion. The mRNAs of adenylyl cyclases and the corresponding proteins were mainly detected in oocytes. Persistent or transient incubation with forskolin induced meiotic resumption in FEOs. The maturation induced by persistent forskolin treatment was inhibited by carbenoxolone. However, carbenoxolone had no effect on the maturation of FEOs transiently treated with forskolin or persistently treated with follicle-stimulating hormone. Oocyte maturation was inhibited by sequential treatment with carbenoxolone followed by forskolin. The carbenoxoloneinduced maturation was accompanied by a cAMP surge, increased PDE3A and MAPK activation, and decreased levels of cGMP and cAMP-dependent PKA I activation.

Keywords
cAMP
cGMP
GJCs
PDE3A
PKA I
MAPK
follicle-enclosed oocyte
FEO
Maturation
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