IMR Press / FBE / Volume 4 / Issue 8 / DOI: 10.2741/E587

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Gene expression in visceral and subcutaneous adipose tissue in overweight women

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1 Department of Endocrinology and Metabolic Disorders, Clinical Hospital Center Zagreb, University Hospital Center Zagreb, Zagreb, Croatia
2 Department for Functional Genomics, Center for Translational and Clinical Research, University of Zagreb School of Medicine, and University Hospital Center Zagreb, Zagreb, Croatia
3 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
4 Department of Hepato-Biliar Surgery and Transplantation of Abdominal Organs, Clinical Hospital Center Zagreb, University Hospital Center Zagreb, Zagreb, Croatia

*Author to whom correspondence should be addressed.

Academic Editor: Nives Pecina-Slaus

Front. Biosci. (Elite Ed) 2012, 4(8), 2734–2744; https://doi.org/10.2741/E587
Published: 1 June 2012
(This article belongs to the Special Issue Biology of brain tumors)
Abstract

Excess weight and obesity are common health problems with multifactorial and polygenic causes. Abdominal or visceral obesity is associated with a higher risk of obesity related complications. The aim of the study was to evaluate differentially expressed genes in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) of 10 overweight women undergoing elective laparoscopic cholecystectomy. Following expression profiling using microarrays, a set of 294 genes that exhibited differential expression between VAT and SAT was further analyzed to test the functional correlation of gene sets using the Gene Set Enrichment Analysis method. To confirm the functional pathways involved in differential expression between SAT and VAT, additional pathway analysis was done using the GeneGo MetaCore software and the Ingenuity Pathway Analysis. Ten differentially expressed genes were selected according to the microarray data, with seven exhibiting significant differential expression in the RT-PCR experiments. The data from this preliminary study suggest enrichment of inflammation and oxidative stress related pathways in VAT, while insulin homeostasis pathways as well as pathways pertaining to several growth factors are enriched in SAT.

Keywords
Visceral adipose tissue
Subcutaneous adipose tissue
Gene expression
Insulin homeostasis
Inflammation pathway
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