IMR Press / FBE / Volume 4 / Issue 7 / DOI: 10.2741/E569

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Identifying lipid metabolism genes in pig liver after clenbuterol administration

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1 State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China
2 College of Life Science and Biotechnology, Hebei Normal University of Science& Technology, Qinhuangdao, 066600, China

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2012, 4(7), 2605–2616; https://doi.org/10.2741/E569
Published: 1 June 2012
Abstract

Clenbuterol is a repartition agent (beta 2-adrenoceptor agonist) that can decrease fat deposition and increase skeletal muscle growth at manageable dose. To better understand the molecular mechanism of Clenbuterol’s action, GeneChips and real-time PCR were used to compare the gene expression profiles of liver tissue in pigs with/without administration of Clenbuterol. Metabolism effects and the global gene expression profiles of liver tissue from Clenbuterol-treated and untreated pigs were conducted. Function enrichment tests showed that the differentially expressed genes are enriched in glycoprotein protein, plasma membrane, fatty acid and amino acid metabolic process, and cell differentiation and signal transduction groups. Pathway mining analysis revealed that physiological pathways such as MAPK, cell adhesion molecules, and the insulin signaling pathway, were remarkably regulated when Clenbuterol was administered. Gene prioritization algorithm was used to associate a number of important differentially expressed genes with lipid metabolism in response to Clenbuterol. Genes identified as differentially expressed in this study will be candidates for further investigation of the molecular mechanisms involved in Clenbuterol’s effects on adipose and skeletal muscle tissue.

Keywords
Microarray
Lipid metabolism
Clenbuterol
Adrenoceptor agonist
Liver
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