IMR Press / FBE / Volume 4 / Issue 7 / DOI: 10.2741/E567

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Expression, identification and purification of human FMRP Isoform 10

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1 Department of endocrinology, National Research Institute for Family Planning, Beijing, 100081, China
2 WHO Collaborative Center for Research in Human Reproduction, Beijing, 100081, China
3 School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, China
4 Pharmaceutical College of Henan University, Kaifeng, 475000, China
5 Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2012, 4(7), 2579–2583; https://doi.org/10.2741/E567
Published: 1 June 2012
Abstract

Fragile X syndrome (FXS), which is the most frequently inherited mental retardation after Down syndrome, is caused by the absence of the fragile X mental retardation protein (FMRP) encoded by the fragile X mental retardation 1 (FMR1) gene. Patients with FXS can be identified by antibody tests that detect the absence of FMRP caused by loss-of-function mutations including the prevalent CGG repeat amplification in lymphocytes. Although the expression of recombinant FMRP in prokaryotic and eukaryotic expression systems has been achieved in different laboratories, the solubilization and purification of this protein is time consuming, varies with each protocol, and often results in low yield. In this study, glutathione S-transferase FMRP fusion protein (GST-FMRP) was expressed in and purified from Escherichia coli BL21(DE3) pLysS cells transformed with pGEX-6P-1 fusion expression vector containing the FMR1 cDNA. The recombinant GST-FMRP was purified on a glutathione sepharose 4B affinity column and detected using SDS-PAGE followed by western blotting with anti-FMRP antibody. This highly purified and soluble GST-FMRP protein can be very beneficial for generating anti-FMRP antibodies and developing FXS diagnosis kits.

Keywords
Fragile X syndrome
FMRP ISO 10
Protein purification
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