IMR Press / FBE / Volume 4 / Issue 7 / DOI: 10.2741/E550

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Adenovirus KH901 promotes 5-FU antitumor efficacy and S phase in LoVo cells

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1 Department of Medical Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University,shanghai 200032, China
2 Chengdu Kanghong Biotechnology Co., Ltd, 36 Xishu Rd, Chengdu, Sichuan 610036, China

*Author to whom correspondence should be addressed.

Academic Editor: Wei Qin

Front. Biosci. (Elite Ed) 2012, 4(7), 2389–2395; https://doi.org/10.2741/E550
Published: 1 June 2012
(This article belongs to the Special Issue Pathogenesis and diagnostic modalities in cancer)
Abstract

A combination of oncolytic and chemotherapeutic agents has been used to kill cancer cells. However, the effect of oncolytic adenoviruses on the cell cycle remains to be determined. Cytotoxicity assays were performed to determine cell death in cells treated with 5-fluorouracil (5- FU) alone or in combination with the oncolytic adenovirus KH901. Dynamic changes in the cell cycle, cell proliferation, and apoptosis-related proteins including pAKT, Bcl-2, Bax, and caspase 3 were investigated after treatment with 5-FU with or without KH901. A higher proportion of S-phase cells were observed after treatment with KH901 and 5-FU than with 5-FU alone. p-AKT, Bcl2, and Bax expression was increased upon treatment with KH901, whereas the expression of caspase-3 was not induced upon treatment with KH901 with or without 5-FU. KH901 exhibited significant potential as an oncolytic adenovirus and increased cell death in combination with 5- FU in LoVo cells, as compared to 5-FU alone. In conclusion, KH901 stimulates LoVo cells to enter the Sphase by activation of p-AKT, which could partly explain its synergistic effect with 5-FU on LoVo cell cytotoxicity.

Keywords
KH901
5-fluorouracil
p-AKT
LoVo cells
Antitumor efficacy
S phase
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