IMR Press / FBE / Volume 3 / Issue 4 / DOI: 10.2741/E347

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
The A640G CYBA polymorphism associates with subclinical atherosclerosis in diabetes
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1 Division of Cardiovascular Sciences, Center for Applied Medical Research, University of Navarra, Spain
2 Department of Internal Medicine, University Clinic, University of Navarra, Spain
3 Department of Cardiology and Cardiovascular Surgery, University Clinic, University of Navarra, Spain

*Author to whom correspondence should be addressed.

Academic Editor: Ana Fortuno

Front. Biosci. (Elite Ed) 2011, 3(4), 1467–1474; https://doi.org/10.2741/E347
Published: 1 June 2011
Abstract

Oxidative stress is implicated in diabetes. The NADPH oxidases are the main source of superoxide in phagocytic and vascular cells, and p22phox is a key subunit. Genetic variants of CYBA, the human p22phox gene, associate with cardiovascular disease. We investigated the association of the A640G polymorphism with diabetes and its impact on phagocytic NADPH oxidase-dependent superoxide production and subclinical atherosclerosis. We studied 1212 subjects in which clinical parameters including carotid intima-media thickness (cIMT) were assessed. The A640G polymorphism was genotyped by TaqMan probes. In 496 subjects, the NADPH oxidase-dependent superoxide production in peripheral blood mononuclear cells was assessed by chemiluminescence. The GG genotype prevalence was significantly higher in type 2 diabetic patients than in non-diabetic subjects. Peripheral blood mononuclear cells from diabetic GG patients presented higher NADPH oxidase-dependent superoxide production than those of diabetic AA/AG patients. Within the diabetic group, GG patients presented higher cIMT levels than AA/AG patients. The A640G CYBA polymorphism may be a marker of oxidative stress risk and may be indicative of subclinical atherosclerosis in type 2 diabetes.

Keywords
Atherosclerosis
Diabetes
Intima-Media Thickness
NADPH oxidase
oxidative stress
A640G polymorphism
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