IMR Press / FBE / Volume 3 / Issue 4 / DOI: 10.2741/E346

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

CD155 is involved in NK-cell mediated lysis of human hepatoblastoma in vitro

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1 Department of Pediatric Oncology, University Children’s Hospital Tuebingen, Hoppe-Seyler-Str.3, 72076 Tuebingen, Germany
2 Department of Pediatric Surgery, University Children’s Hospital Tuebingen, Hoppe-Seyler-Str.3, 72076 Tuebingen, Germany
3 Institute of Pathology, 71229 Leonberg, Germany
4 Department II of Internal Medicine, Section of Transplantation Immunology and Immunohematology, University Hospital, Otfried-Mueller Strasse 10, D-72076 Tuebingen, Germany
5 Department of Immunology, Institute for Cell Biology, University of Tuebingen, Auf der Morgenstelle 15, 72076 Tuebingen

*Author to whom correspondence should be addressed.

Academic Editor: Steven Warmann

Front. Biosci. (Elite Ed) 2011, 3(4), 1456–1466; https://doi.org/10.2741/E346
Published: 1 June 2011
(This article belongs to the Special Issue Hepatoblastoma)
Abstract

NK cells are involved in the lysis of different solid tumors and leukemias. NK-activity is thereby regulated by activating and inhibitory receptors. Until now, nothing is known about the NK-activity against hepatoblastoma and the involved receptors. We tested NK cells for cytotoxicity against HB in vitro. Expression levels of activating NK ligands were analysed on 13 primary HB samples as well as on 3 HB cell lines. All HB cell lines showed low HLA-class-I-expression. CD155 expression was strong on primary HB samples and cell lines. NKG2D-ligands (MICA/B, ULBP1-3) were heterogeneous expressed in primary samples and cell cultures. There were no differences between the various histological subtypes. NK cells showed strong cytotoxicity in vitro which was significantly increased through interleukin-2 and –15 stimulation (p<.001). Blockade of CD155 resulted in decreased lysis rates. Our findings show that NK cells exert high activity against hepatoblastoma in vitro and that CD155 is involved in the NK mediated killing of HB. The inclusion of a NK-based immunotherapy into novel treatment strategies might be a promising alternative especially for advanced tumors.

Keywords
Hepatoblastoma
NK-cells
Immunotherapy
CD155
Interleukin-2
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