IMR Press / FBE / Volume 3 / Issue 4 / DOI: 10.2741/E336

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Separation of anti-neoplastic activities by fractionation of a Pluchea odorata extract

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1 Institute of Clinical Pathology, Medical University of Vienna, Waehringer Guertel 18-20, Austria
2 Department of Pharmacognosy, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, Austria
3 Department of Botany, Museum of Natural History, Burgring 7, A-1010 Vienna, Austria
4 Department of Nutritional Sciences, University of Vienna, Althanstrasse 14, Austria
5 Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Waehringer Guertel 18-20, Austria
6 Institute for Ethnobiology, Playa Diana, San Jose/Peten, Guatemala
7 Finzelberg GmbH & Co. KG, Koblenzer Strasse 48-54, D-56626 Andernach, Germany

*Author to whom correspondence should be addressed.

Academic Editor: Hala Gali-Muhtasib

Front. Biosci. (Elite Ed) 2011, 3(4), 1326–1336; https://doi.org/10.2741/E336
Published: 1 June 2011
(This article belongs to the Special Issue New emerging anticancer lead compounds from plants)
Abstract

Natural products continue to represent the main source for therapeutics, and ethnopharmacological remedies from high biodiversity regions are a rich source for the development of novel drugs. Hence, in our attempt to find new anti-neoplastic activities we focused on ethno-medicinal plants of the Maya, who live in the world's third richest area in vascular plant species. Pluchea odorata (Asteraceae) is traditionally used for the treatment of various inflammatory disorders and recently, the in vitro anti-cancer activities of different extracts of this plant were described. Here, we present the results of bioassay-guided fractionations of the dichloromethane extract of P. odorata that aimed to enrich the active principles. The separation resulted in fractions which showed the dissociation of two distinct anti-neoplastic mechanisms; firstly, a genotoxic effect that was accompanied by tubulin polymerization, cell cycle arrest, and apoptosis (fraction F2/11), and secondly, an effect that interfered with the orchestrated expression of Cyclin D1, Cdc25A, and Cdc2 and that also led to cell cycle arrest and apoptosis (fraction F3/4). Thus, the elimination of generally toxic properties and beyond that the development of active principles of P. odorata, which disturb cancer cell cycle progression, are of interest for potential future therapeutic concepts against proliferative diseases.

Keywords
ASE accelerated solvent extractor
ASR anisaldehyde sulphuric acid reagent
CC-I column chromatography I
CC-II column chromatography II
CHCl3 chloroform
CH2Cl2 dichloromethane
SDSPAGE sodiumdodecylsulfonate polyacrylamide gel electrophoresis
PE petroleum ether
PIC Protease Inhibitor Cocktail
PMSF phenylmethylsulfonylfluorid
TLC thin layer chromatography
VLC vacuum liquid chromatography
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