IMR Press / FBE / Volume 3 / Issue 3 / DOI: 10.2741/E309

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Prune melanoidins protect against oxidative stress and endothelial cell death
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1 Department of Biomedical Sciences, Centre of Excellence for Biotechnology Development and Biodiversity Research
2 National Institute of Biostructures and Biosystems, Viale San Pietro 34/B, 07100 Sassari, Italy
3 Department of Agricultural Environmental Sciences and Food Biotechnology, University of Sassari, Viale Italia, 39, 07100 Sassari, Italy
4 Department of Physiological, Biochemical and Cellular Science, University of Sassari, Via Muroni 25, 07100 Sassari, Italy

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2011, 3(3), 1034–1041; https://doi.org/10.2741/E309
Published: 1 June 2011
Abstract

The health-promoting effects of fruit and vegetable consumption are thought to be due to phytochemicals contained in fresh plant material. Whether processed plant foods provide the same benefits as unprocessed ones is an open question. Melanoidins from heat-processed plums (prunes) were isolated and their presence confirmed by hydroxymethylfurfural content and browning index. Oxidative-induced endothelial cell (EC) damage is the trigger for the development of cardiovascular diseases (CVD); therefore the potential protective effect of prune melanoidins on hydrogen peroxide-induced oxidative cell damage was investigated on human endothelial ECV304 cells. Cytoplasmic and mitochondrial redox status was assessed by using the novel, redox-sensitive, ratiometric fluorescent protein sensor (roGFP), while mitochondrial membrane potential (MMP) was investigated with the fluorescent dye, JC-1. Treatment of ECV304 cells with hydrogen peroxide dose-dependently induced both mitochondrial and cytoplasmic oxidation, in addition to MMP dissipation, with ensuing cell death. Pretreatment of ECV304 with prune melanoidins, significantly counteracted and ultimately abolished hydrogen peroxide elicited phenomena, clearly indicating that these polymers protect human EC against oxidative stress.

Keywords
Maillard Reaction Products
Prunes
Melanoidins
Reactive Oxygen Species
Antioxidant
Endothelial Cell Protection
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