IMR Press / FBE / Volume 3 / Issue 3 / DOI: 10.2741/E307

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Development of semiconductor nanomaterial whole cell imaging sensor on glass slides
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1 State Key Laboratory of Food Science and Technology, Jiangxi-OAI Joint research institute, Nanchang University, 235 Nanjing Donglu, Nanchang 330047, China
2 Ocean NanoTech LLC, 2143 Worth Lane, Springdale AR 72764, USA

*Author to whom correspondence should be addressed.

Academic Editor: Zoraida P. Aguilar

Front. Biosci. (Elite Ed) 2011, 3(3), 1013–1024; https://doi.org/10.2741/E307
Published: 1 June 2011
(This article belongs to the Special Issue Nano and bio sensors)
Abstract

We report the development of a highly specific semiconductor quantum dots (QDs)-based whole cell imaging sensor that offer rapid, reproducible, accurate, and long term cell imaging system on silanized microscope glass slides. The QD-based imaging sensor involved capture of whole cells with QD labeled highly specific antibodies against over expressed cell membrane proteins. The QDs were first modified with a polymer coating to generate carboxyl groups on the surface. Using the carboxylated QDs, antibodies were covalent conjugated using carbodiimide chemistry to form 20Ab~QD that were used to capture whole cell. The SK-BR3 cell line was used as a model analyte in the sandwich type assay consisting of 10Ab + SK-BR3 + Ab' + 20Ab~QD. The assay was immobilized on an antibody modified silanized microscope slide that was subsequently mounted on a fluorescence microscope for detection. The results indicated that the QD based imaging sensor exhibited brighter signals compared with organic dye Texas red. The QD-based whole cell imaging sensor was visible under the microscope even after one week without fixation.

Keywords
Quantum Dots
QDs
Whole Cell
Cell
Semiconductor Nanomaterials
Biosensor
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